Natural polyhydroxyalkanoate-gold nanocomposite based biosensor for detection of antimalarial drug artemisinin.
Identifieur interne : 000106 ( Main/Exploration ); précédent : 000105; suivant : 000107Natural polyhydroxyalkanoate-gold nanocomposite based biosensor for detection of antimalarial drug artemisinin.
Auteurs : RBID : pubmed:24582254Abstract
The worrisome trend of antimalarial resistance has already highlighted the importance of artemisinin as a potent antimalarial agent. The current investigation aimed at fabricating a biosensor based on natural polymer polyhydroxyalkanoate-gold nanoparticle composite mounting on an indium-tin oxide glass plate for the analysis of artemisinin. The biosensor was fabricated using an adsorbing horse-radish peroxidase enzyme on the electrode surface for which cyclic voltammetry was used to monitor the electro-catalytic reduction of artemisinin under diffusion controlled conditions. Electrochemical interfacial properties and immobilization of enzyme onto a polyhydroxyalkanoate-gold nanoparticle film were evaluated, and confirmed by cyclic voltammetry, electrochemical impedance spectroscopy and scanning electron microscopy. The differential pulse voltammetric peak current for artemisinin was increased linearly (concentration range of 0.01-0.08μg mL(-1)) with sensitivity of 0.26μAμg mL(-1). The greater sensitivity of the fabricated biosensor to artemisinin (optimum limits of detection were 0.0035μg mL(-1) and 0.0036μg mL(-1) in bulk and spiked human serum, respectively) could be of much aid in medical diagnosis.
DOI: 10.1016/j.msec.2014.01.019
PubMed: 24582254
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Natural polyhydroxyalkanoate-gold nanocomposite based biosensor for detection of antimalarial drug artemisinin.</title><author><name sortKey="Phukon, Pinkee" uniqKey="Phukon P">Pinkee Phukon</name><affiliation wicri:level="1"><nlm:affiliation>Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur 784028, Assam, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur 784028, Assam</wicri:regionArea></affiliation></author><author><name sortKey="Radhapyari, Keisham" uniqKey="Radhapyari K">Keisham Radhapyari</name><affiliation wicri:level="1"><nlm:affiliation>Analytical Chemistry Division, CSIR-North East Institute of Science & Technology, Jorhat 785006, Assam, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Analytical Chemistry Division, CSIR-North East Institute of Science & Technology, Jorhat 785006, Assam</wicri:regionArea></affiliation></author><author><name sortKey="Konwar, Bolin Kumar" uniqKey="Konwar B">Bolin Kumar Konwar</name><affiliation wicri:level="1"><nlm:affiliation>Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur 784028, Assam, India; Nagaland University (Central), Lumami, Zunheboto, Nagaland 798627, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur 784028, Assam, India; Nagaland University (Central), Lumami, Zunheboto, Nagaland 798627</wicri:regionArea></affiliation></author><author><name sortKey="Khan, Raju" uniqKey="Khan R">Raju Khan</name><affiliation wicri:level="1"><nlm:affiliation>Analytical Chemistry Division, CSIR-North East Institute of Science & Technology, Jorhat 785006, Assam, India. Electronic address: khan.raju@gmail.com.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Analytical Chemistry Division, CSIR-North East Institute of Science & Technology, Jorhat 785006, Assam</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><date when="2014">2014</date><idno type="doi">10.1016/j.msec.2014.01.019</idno><idno type="RBID">pubmed:24582254</idno><idno type="pmid">24582254</idno><idno type="wicri:Area/Main/Corpus">000139</idno><idno type="wicri:Area/Main/Curation">000139</idno><idno type="wicri:Area/Main/Exploration">000106</idno></publicationStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The worrisome trend of antimalarial resistance has already highlighted the importance of artemisinin as a potent antimalarial agent. The current investigation aimed at fabricating a biosensor based on natural polymer polyhydroxyalkanoate-gold nanoparticle composite mounting on an indium-tin oxide glass plate for the analysis of artemisinin. The biosensor was fabricated using an adsorbing horse-radish peroxidase enzyme on the electrode surface for which cyclic voltammetry was used to monitor the electro-catalytic reduction of artemisinin under diffusion controlled conditions. Electrochemical interfacial properties and immobilization of enzyme onto a polyhydroxyalkanoate-gold nanoparticle film were evaluated, and confirmed by cyclic voltammetry, electrochemical impedance spectroscopy and scanning electron microscopy. The differential pulse voltammetric peak current for artemisinin was increased linearly (concentration range of 0.01-0.08μg mL(-1)) with sensitivity of 0.26μAμg mL(-1). The greater sensitivity of the fabricated biosensor to artemisinin (optimum limits of detection were 0.0035μg mL(-1) and 0.0036μg mL(-1) in bulk and spiked human serum, respectively) could be of much aid in medical diagnosis.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="In-Process"><PMID Version="1">24582254</PMID><DateCreated><Year>2014</Year><Month>03</Month><Day>03</Day></DateCreated><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-0191</ISSN><JournalIssue CitedMedium="Internet"><Volume>37</Volume><PubDate><Year>2014</Year><Month>Apr</Month><Day>1</Day></PubDate></JournalIssue><Title>Materials science & engineering. C, Materials for biological applications</Title><ISOAbbreviation>Mater Sci Eng C Mater Biol Appl</ISOAbbreviation></Journal><ArticleTitle>Natural polyhydroxyalkanoate-gold nanocomposite based biosensor for detection of antimalarial drug artemisinin.</ArticleTitle><Pagination><MedlinePgn>314-20</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.msec.2014.01.019</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0928-4931(14)00027-7</ELocationID><Abstract><AbstractText>The worrisome trend of antimalarial resistance has already highlighted the importance of artemisinin as a potent antimalarial agent. The current investigation aimed at fabricating a biosensor based on natural polymer polyhydroxyalkanoate-gold nanoparticle composite mounting on an indium-tin oxide glass plate for the analysis of artemisinin. The biosensor was fabricated using an adsorbing horse-radish peroxidase enzyme on the electrode surface for which cyclic voltammetry was used to monitor the electro-catalytic reduction of artemisinin under diffusion controlled conditions. Electrochemical interfacial properties and immobilization of enzyme onto a polyhydroxyalkanoate-gold nanoparticle film were evaluated, and confirmed by cyclic voltammetry, electrochemical impedance spectroscopy and scanning electron microscopy. The differential pulse voltammetric peak current for artemisinin was increased linearly (concentration range of 0.01-0.08μg mL(-1)) with sensitivity of 0.26μAμg mL(-1). The greater sensitivity of the fabricated biosensor to artemisinin (optimum limits of detection were 0.0035μg mL(-1) and 0.0036μg mL(-1) in bulk and spiked human serum, respectively) could be of much aid in medical diagnosis.</AbstractText><CopyrightInformation>Copyright © 2014 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Phukon</LastName><ForeName>Pinkee</ForeName><Initials>P</Initials><Affiliation>Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur 784028, Assam, India.</Affiliation></Author><Author ValidYN="Y"><LastName>Radhapyari</LastName><ForeName>Keisham</ForeName><Initials>K</Initials><Affiliation>Analytical Chemistry Division, CSIR-North East Institute of Science & Technology, Jorhat 785006, Assam, India.</Affiliation></Author><Author ValidYN="Y"><LastName>Konwar</LastName><ForeName>Bolin Kumar</ForeName><Initials>BK</Initials><Affiliation>Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur 784028, Assam, India; Nagaland University (Central), Lumami, Zunheboto, Nagaland 798627, India.</Affiliation></Author><Author ValidYN="Y"><LastName>Khan</LastName><ForeName>Raju</ForeName><Initials>R</Initials><Affiliation>Analytical Chemistry Division, CSIR-North East Institute of Science & Technology, Jorhat 785006, Assam, India. Electronic address: khan.raju@gmail.com.</Affiliation></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType>Journal Article</PublicationType><PublicationType>Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>01</Month><Day>15</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Mater Sci Eng C Mater Biol Appl</MedlineTA><NlmUniqueID>101484109</NlmUniqueID><ISSNLinking>0928-4931</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Artemisinin</Keyword><Keyword MajorTopicYN="N">Biopolymer</Keyword><Keyword MajorTopicYN="N">Biosensors</Keyword><Keyword MajorTopicYN="N">Electrochemical impedance spectroscopy</Keyword><Keyword MajorTopicYN="N">Horse-radish peroxidase</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2013</Year><Month>3</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2013</Year><Month>11</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2014</Year><Month>1</Month><Day>8</Day></PubMedPubDate><PubMedPubDate PubStatus="aheadofprint"><Year>2014</Year><Month>1</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>3</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>3</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>3</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pii">S0928-4931(14)00027-7</ArticleId><ArticleId IdType="doi">10.1016/j.msec.2014.01.019</ArticleId><ArticleId IdType="pubmed">24582254</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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